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KMID : 1234420110390040324
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Korean Journal of Microbiololgy and Biotechnology
2011 Volume.39 No. 4 p.324 ~ p.329
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Plant Growth Promotion Activity of Endophytic Fungi Isolated from the Roots of Calystegia soldanella
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You Young-Hyun
Yoon Hyeok-Jun
Woo Ju-Ri
Seo Yeonggyo
Kim Jong-Guk
Shin Jae-Ho
Lee In-Jung
Choo Yeon-Sik
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Abstract
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Eight endophytic fungal strains were isolated from the roots of Calystegia soldanella from the western coast of South Korea. The culture filtrate of the eight endophytic fungi were applied to waito-c rice seedlings in order to verify potential plant growth promotion activities. The results of bioassay indicated that the Cs-9-7 fungal strain possessed the highest plant growth promotion activity. Fungal culture filtrates were analyzed to verify secondary metabolites using gas chromatography and mass spectroscopy with selected ion monitoring (GC/MS-SIM). The culture filtrate of the Cs-9-7 fungal strain was confirmed to contain gibberellins GA3 (1.229 ng/mL), GA4 (3.535 ng/mL), GA7 (1.408 ng/mL) and GA12 (0.378 ng/mL). Polymerase chain reactions (PCR) were performed so as to determine the internal transcribed spacer (ITS) regions for the identification of isolated strains with universal primers ITS-1 and ITS-4. The Cs-9-7 fungal strain, isolated from the root of C. soldanella, has been named Aspergillus tubingensis Cs-9-7.
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KEYWORD
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Aspergillus tubingensis, Calystegia soldanella, endophytic fungi, gibberellin, plant growth promotion
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